SOCS1 anticorps (C-Term)

N° du produit ABIN374072

L’anticorps Chèvre Polyclonal anti-SOCS1 a été validé pour WB, IHC (p) et EIA. Il convient pour détecter SOCS1 dans des échantillons de Humain, Souris, Rat, Boeuf (Vache) et Chien.

Aperçu rapide pour SOCS1 anticorps (C-Term) (ABIN374072)

Antigène: SOCS1 (Suppressor of Cytokine Signaling 1 (SOCS1))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp SOCS1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-SOCS1 anticorps

Épitope: C-Term

Séquence: C-VLRDYLSSFP FQI

Specificité: Recognizes Suppressor Of Cytokine Signaling 1 (SOCS1) at C-term.

Réactivité croisée (Details): Species reactivity (expected):Rat, Canine (Dog) and Bovine.
Species reactivity (tested):Human, Mouse.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogène: Synthetic peptide from the C-Terminus of Human SOCS1 (NP_003736.1). Genename: SOCS1

Information d'application

Indications d'application: Peptide ELISA: Limit Dilution: 1/4000. Western blot: 1-3 μg/mL. In transfected HEK293 transiently expressing human SOCS1 bandsof approx.30 kDa and 20 kDa are observed. These bands are not observed in thenon-transfected HEK293. A similar pattern is observed in Mouse Spleen lysates. Thecalculated molecular size is 23.0 kDa according to Human NP_003736.1 and 23.0 kDa

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline buffer, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur SOCS1

Antigène: SOCS1 (Suppressor of Cytokine Signaling 1 (SOCS1))

Autre désignation: SOCS1

Sujet: SOCS1 represents a family of proteins that regulates responses to cytokines. The SOCS family comprises at least 8 members, SOCS1 to SOCS7 and CIS (cytokine inducible SH2 containing protein). Structurally, the SOCS proteins are composed of an N terminal region of variable length and amino acid composition, a central SH2 domain, and a previously unrecognized C terminal motif named the SOCS box. SOCS1 (also known as Janus kinase binding protein and STAT induced STAT inhibitor 1) was isolated independently by its ability to interact with Janus kinase 2 in a 2 hybrid screen and on the basis of antigenic similarity with the SH2 domain of STAT3. SOCS 1 inhibition of signal transduction appears to occur by binding to and inhibiting the catalytic activity of members of JAK family of cytoplasmic kinases (direct JAK2 kinase inhibition). Biochemical characterization as well as gene disruption studies indicate that SOCS1 is an important negative regulator of JAK-STAT signal pathway.Synonyms: JAB, JAK-binding protein, SOCS-1, SSI-1, SSI1, STAT-induced STAT inhibitor 1, Suppressor of cytokine signaling 1, TIP-3, TIP3, Tec-interacting protein 3

ID gène: 8651

NCBI Accession: NP_003736

UniProt: O15524

Pathways: Signalistation JAK/STAT, Interferon-gamma Pathway, Signalisation TLR, Response to Growth Hormone Stimulus